Xenopus tropicalis (Gray, 1864) homotypic synonym: Xenopus (Silurana) tropicalis. Jump to: navigation, search. Garland Publishing Inc, New York ISBN 0-8153-1896-0. Pedro's BioMolecular Research Tools A Collection of WWW Links to Information and Services Useful to Molecular Biologists. Xenopus laevis and Xenopus tropicalis have been used extensively in developmental and cell biology, biochemistry, functional genomics and immunology. Procedures input by VGP link to Kroll lab Xenopus environmental procedure SOP document at Washington University animal facility: Modified format below: A. XenOrtho DB (Xenopus Ortholog DataBase) is a database in which ortholog genes of Xenopus tropicalis, Xenopus laevis and humans are listed. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure to water to spermatozoa of some aquatic species. Silurana tropicalis Gray, 1864. drs6j@virginia.edu The mesoderm, comprising the tissues that come to lie entirely in the deep layer, … Xenopi cannot raise their body temperature by producing metabolic heat. Session et al. Shook DR(1), Majer C, Keller R. Author information: (1)Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, VA 22903, USA. This is the first amphibian genome to be sequenced, enabling new insights into vertebrate evolution and the last common ancestor of tetrapods. XDB is a Web data catalogue of NIBB/NIG Xenopus laevis EST project. Axeldb A database focussing on gene expression in the frog Xenopus laevis. X. laevis frogs are larger than X. tropicalis frogs and so are their cells, eggs, DNA content, and nuclei. Xenopus contains at least three sarcomeric alpha actin genes that are preferentially expressed in either heart or skeletal muscle. Verlag der Zeitschrift für Naurforschung, Germany Google Scholar. Lateral line systems, usually found in fish, detect vibrations in the water. Function i. It uses extremely sensitive fingers, an acute sense of smell, and its lateral line systems to locate food. Robust and efficient protocols for fertilization and early embryo care of Xenopus laevis and Xenopus tropicalis are essential for experimental success, as well as maintenance and propagation of precious animal stocks. (2016) have sequenced the genome of Xenopus laevis. Digitized images and developmental data from Nieuwkoop and Faber (1994) Normal Table of Xenopus laevis (Daudin). A transgenic line of X. tropicalis was used to examine the process of cornea-lens transdifferentiation in which green fluorescent protein (GFP) is expressed in differentiated lens cells under the control of the Xenopus gamma1-crystallin promoter element. al. The rapid growth of the National Xenopus Resource has required effective implementation and optimization of these protocols. Introduction. Genetic Map of Xenopus tropicalis Jamie Davies The kidney development database. Room temperature (controlled automatically by building … They must rely on external heat sources. Likewise, X. tropicalis has been selected over X. laevis as the subject of a complete genome sequencing project for the same reason. Like Xenopus laevis, X. tropicalis was formerly used in pregnancy tests (Bergerard & Lamotte 1949). However, X. laevis has an allotetraploid genome precluding its use in forward genetic analysis. Xenopus commonly known as the clawed frog) is a genus of aquatic frogs native to sub-Saharan Africa.. Twenty species are known. Available as frozen sperm, please allow 8 months for live animals. Xenopus laevis tropicalis. Kalt MR (1976) Morphology and kinetics of spermatogenesis in Xenopus laevis. Xenopus tropicalis (Gray, 1864) basionym: Silurana tropicalis Gray, 1864. homotypic synonym: Xenopus (Silurana) tropicalis. It has a voracious appetite and attacks anything that passes in front of it. Phosphatase which catalyzes the conversion of NADP(+) to NAD(+) and of NADPH to NADH (By similarity). Component of the circadian clock or downstream effector of clock function (PubMed:12573214). Xenopus egg extract is a lysate that is prepared by crushing the eggs of the African clawed frog Xenopus laevis.It offers a powerful cell-free (or in vitro) system for studying various cell biological processes, including cell cycle progression, nuclear transport, DNA replication and chromosome segregation.It is also called Xenopus egg cell-free system or Xenopus egg cell-free extract. Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. Life cycle, evolutionary position and regenerative potential of Xenopus. … Probable transcription factor. It was produced by Dr. Fukui (Faculty of Science and Engineering, Chuo Univ.) Xenopus tropicalis has been developed as a model organism for developmental biology, ... Three ngn subfamilies (Ngnr1–3) have been isolated in both Xenopus laevis and Xenopus tropicalis (Nieber et al., 2009). Xenopus Laevis should be housed at 20-23 degrees C (68-75 degrees F). Due to the tetraploid nature of Xenopus laevis, each of these three alpha actin genes is present in at least two copies. *Significantly greater or less (p < 0.05) than the corresponding values for controls. The word Xenopus means "strange foot" and laevis means "smooth". Exhibits a high amplitude circadian rhythm with maximal levels in early evening (PubMed:12573214). A small, fast‐breeding, diploid relative of the frog Xenopus laevis, Xenopus tropicalis, has recently been adopted for research in developmental genetics and functional genomics.X. amphibians, which include Xenopus laevis (X. laevis) and Xenopus tropicalis (X. tropicalis), are one such group of animals with high regenerative capacity (Dinsmore, 1991). From the early 1700s to the present, Xenopus have been studied as animal … Xenopus laevis tropicalis. Ngn1 is expressed in the otic placode at early embryological stages (up to NF22), but with somewhat stronger expression in Xenopus tropicalis than in Xenopus laevis. Xenopus laevis (XLA) is an allotetraploid species which appears to have undergone whole-genome duplication after the interspecific hybridization of 2 diploid species closely related to Silurana/Xenopus tropicalis (XTR). Reviewed-Annotation score: -Experimental evidence at transcript level i. tropicalis shares advantages of X. laevis as a classic embryologic system, but its simpler genome and shorter generation time make it more convenient for multigenerational genetic, genomic, and transgenic … Carruthersa, S., et. Room Environmental Parameters. The genome of X. tropicalis has been sequenced (Hellsten et al. Genetic analysis may be applicable to Xenopus (Silurana) tropicalis, which has a diploid genome and a s … Experiments were repeated three times. Xenopus Tropicalis should be housed at 23-28 degrees C (75 -80 degrees F). Xenopus tropicalis was somewhat less sensitive to 6‐AN, semicarbizide and atrazine when tested at 27 °C than at 23 °C. Shows a small preference for NADPH over NADP(+) (By similarity). Reference: EXRC. Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. Introduction. The African Clawed Frog (Xenopus laevis) is also known to tropical fish keeping enthusiasts as the Xenopus, African Clawed Toad, or African Claw Toed frog and is the only amphibian that actually has claws. Xenopus laevis is a scavenger and eats living, dead, or dying arthropods and other pieces of organic waste. Because X. tropicalis is diploid and X. laevis is tetraploid, important inferences can be made about genome evolution. We have demonstrated here that monospermy in the clawed frog, Xenopus tropicalis, as well as in X. laevis, is ensured by a fast, electrical block to polyspermy on the egg plasma membrane after the entry of the first sperm, which is mediated by the positive-going fertilization potential. Xenopus laevis and Xenopus tropicalis have long been used to drive discovery in developmental, cell, and molecular biology. If the water temperature is sub optimal... frogs will not eat and their metabolism and immune systems will be depressed. However, another species of frog Xenopus tropicalis can also be a source. The early development of Xenopus Laevis, an atlas of the histology. Pattern and morphogenesis of presumptive superficial mesoderm in two closely related species, Xenopus laevis and Xenopus tropicalis. The cardiac versus skeletal expression patterns of actins are probably sequence-dependent. 26. X. laevis is the species of frog most often used to generate egg extracts. 1. Xenopus laevis (African clawed frog) Status. Xenopus tropicalis, on the other hand, possesses a diploid genome and a short generation time, ... (C and D) in stage 55 Xenopus laevis and Xenopus tropicalis tadpoles. 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